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KMID : 0829320160190030077
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Korean Journal of Clinical Microbiology
2016 Volume.19 No. 3 p.77 ~ p.81
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Rapid Diagnosis of Mycobacterium abscessus Bacteremia Using Matrix-Assisted Laser Desorption Ionization Time-of-Flight Mass Spectrometry
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Won Eun-Jeong
Choi Yong-Jun
Kim Soo-Hyun
Shin Jong-Hee
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Abstract
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Mycobacterium abscessus was isolated from cultures of seven blood samples from a 64-year-old diabetic female who was admitted due to steroid-unresponsive adrenal insufficiency. The isolates were difficult to identify using the conventional commercial systems, VITEK 2 (bioMerieux, France) or MicroScan (Siemens Healthcare Diagnostics, USA), but were rapidly identified as M. abscessus by a matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS)-based Bruker Biotyper system (Bruker Daltonics, USA). Identification of M. abscessus was confirmed by a reverse hybridization- based assay (Genotype Mycobacterium CM/AS 12, Hain Lifescience) and direct sequencing of a heat- shock protein gene. After removal of her central venous catheter, the patient was successfully treated with a combination therapy comprising clarithromycin, amikacin, cefoxitin, and imipenem. Our findings demonstrate that MALDI-TOF MS can facilitate rapid and accurate identification of M. abscessus from blood cultures, which enables prompt administration of appropriate therapy following catheter removal.
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KEYWORD
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Bacteremia, MALDI-TOF, Mycobacterium abscessus
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